Increasing Stability of Cellulase, Obtained from Bacillus subtilis ITBCCB148 with Chemical Modification Using p-Nitrophenolcarbonate-Polyethylenglycol (NPC-PEG)
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چکیده
منابع مشابه
The Chemical Modification of α-Amylase from Locale Bacteria of Bacillus subtilis ITBCCB148 using Citraconic Anhydride
Chemical modification of α-amylase from locale bacteria isolate Bacillus subtilis ITBCCB148 using citraconic anhydride has been performed. increase thermal stability of the enzyme has successfully been done. The results showed that the modified enzymes with citraconic anhydride with modification degree of 45, 73, 82, 83, and 88% have similar optimum pH of 5.5 and optimum temperature of 60°C; ki...
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Chemical modification of α-amylase from locale bacteria isolate Bacillus subtilis ITBCCB148 using glyoxylic acid to increase thermal stability of the enzyme has successfully been done. The results showed that the native and the modified enzyme have similar pH of 6.0 and optimum temperature of 60°C. However, the thermal stability of the modified enzymes was increased 1.8-2.1 times compared to th...
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Cellulases participate in a number of biological events, such as plant cell wall remodelling, nematode parasitism and microbial carbon uptake. Their ability to depolymerize crystalline cellulose is of great biotechnological interest for environmentally compatible production of fuels from lignocellulosic biomass. However, industrial use of cellulases is somewhat limited by both their low catalyt...
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N-Bromosuccinimide completely inactivated the cellulase, and titration experiments showed that oxidation of one tryptophan residue per cellulase molecule coincided with 100% inactivation. CM-cellulose protected the enzyme from inactivation by N-bromosuccinimide. The cellulase was inhibited by active benzyl halides, and reaction with 2-hydroxy-5-nitrobenzyl bromide resulted in the incorporation ...
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In bribery an external agent tries to alter the outcome of an election by changing some voters’ votes. Usually, when investigating bribery problems, full information is assumed, i.e., that the manipulative agent knows the set of candidates, each voter’s votes and the voting rule used. In this paper, we formally introduce different structures of partial information, we show the connections betwe...
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ژورنال
عنوان ژورنال: Oriental Journal of Chemistry
سال: 2017
ISSN: 0970-020X,2231-5039
DOI: 10.13005/ojc/330547